The use of bone marrow derived mesenchymal stem cell for cornea regeneration in rabbit model
Aim or Purpose: To evaluate the use of autologous bone marrow derived mesenchymal stem cells (BM-MSCs) to treat cornea stromal defect in a rabbit model.
Methods: A non-randomized interventional controlled animal study involving twenty one adult New Zealand white rabbits. Corneal deep lamellar dissections were created in three groups of rabbits and treated accordingly. Group I; Autologous bone marrow derived MSCs with autologous fibrin and human amniotic membrane. Group II; Autologous fibrin with human amniotic membrane without MSCs. Group III; No treatment. Clinical outcome was evaluated by corneal re-epithelization, corneal opacity, corneal thickness and histology.
Results: BM-MSCs were successfully isolated from bone marrow of seven rabbits based on the adherence property of the cells to the plastic of the cell culture plate. At day 60 corneal thicknesses was significantly thicker in Group I. The localization of PKH26 labeled BM-MSCs showed an increase in cell density at the transplanted site, proving its role in cornea stromal regeneration. Although the cornea clarity was not achieved in this study, we believe that cornea stromal remodeling requires many months to years to regain its original optical quality.
Conclusion: Locally transplanted BM-MSCs may be a useful source for cornea stromal regeneration. The use of autologous BM-MSCs offers a promising option for treating corneal disorder without the risk of immune-rejection and calcification.
2. Flavia LB, Shyam SC, Alicia C, et al. Corneal myofibroblast generation from bone marrow-derived cells. Exp Eye Res 2010;91:92-96.
3. Chan T, Payor S, Holden BA. Corneal thickness profiles in rabbits using an ultrasonic pachometer. Invest Ophth Vis Sci 1983;24:1408-1410.
4. Fantes FE, Hanna KD, Waring GO, et al. Wound healing after excimer laser keratomileusis (photorefractive keratectomy) in monkeys. Arch Ophthalmol 1990;108:665-675.
5. Gu S, Xing C, Han J, Tso MOM, Hong J. Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo. Mol Vis 2009;15: 99.
6. Yosuke H, Waka I, Ken F, et al. Identification of keratocyte-like cells differentiated from circulating bone marrow-derived cells in the mouse cornea. Med Mol Morphol 2013;46:233-238.
7. Kamran H, Alexander IK, Irina YP, et al. Monitoring of rabbit cornea response to dehydration stress by optical coherence tomography. Invest Ophth Vis Sci 2004;45:2555-2562.
8. Yanling M, Yongsheng X, Zhifeng X, et al. Reconstruction of chemically burned rat corneal surface by bone marrow–derived human mesenchymal stem cells. Stem Cells 2006;24:315-321.
9. Norzana AG, Ropilah AR, Jemaimah C, et al. Rabbit limbal epithelial cells maintain its stemness in serum-free and feeder layer-free culture system. Tissue Eng Regen Med 2007;4:557-565.
10. Joo YO, Mee KK, Mi SS, et al. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells 2008;26:1047-1055.
11. Soo HP, Kyoung WK, Yeoun SC, Jae CK. Human mesenchymal stem cells differentiate into keratocyte-like cells in keratocyte-conditioned medium. Exp Eye Res 2012;101:16-26.
12. Rohaina CM, Then KY, Angela NMH, et al. Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane. Transl Res 2014;163:200-210.
13. Wakitani S, Goto T, Pineda SJ, et al. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am 1994;76:579-592.
14. Ye J, Yao K, Kim JC. Mesenchymal stem cell transplantation in a rabbit corneal alkali burn model: engraftment and involvement in wound healing. Eye 2005;20:482-490.
Copyright (c) 2016 Asian Journal of Ophthalmology
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication, with the work twelve (12) months after publication simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).